Wednesday, Mar 12, 2014
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In the above example, both enzymes produce tetranucleotides CCGG which can anneal to one another. However, resulting DNA sequence will be:
where the nucleotides shown in italic originate from NotI-cut site, and those in bold from Bsp120I-cut one. Note that the resulting sequence is not recognised by either of the two enzymes.
Other examples of isocaudomers include:
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